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Toxicology

Thin Layer Chromatography

Yoknapatawpha County Forensic Laboratory

DATE ANALYSIS PERFORMED:
5/11/2015

Madeline Holland, Ph.D.
555 Jackson Avenue
Oxford, MS 38655
662-234-XXXX (FAX 662-234-XXXX)

Analysis:Toxicological Analysis

 

REPORT OF FORENSIC TOXICOLOGICAL ANALYSIS

Case Description:
 Barbara Dubois Homicide

Case #
 001347-02E-2015

Investigative Agency:
 Yoknapatawpha County Sheriff's Department

Investigating Officer(s):
 Det. S. Murphy, Det. T. Armstrong

EVIDENCE ANALYZED

  • 001347-06 and 001347-07: Two (2) samples from possible vomit pool in hallway outside YCCC Room 273
  • 001347-22: One (1) medium-sized plastic bag labeled "YCCC Dry Cleaning"
    • Contents: semi-liquid substance visually consistent with vomit

Evidence items were extracted with 5% methanol/chloroform and the organic layer was evaporated to afford a residue, which was subjected to thin layer chromatography (TLC). Control samples included emetine (an active emetic alkaloid found in Psychotria ipecacuanha) and Syrup of Ipecac, USP, from Roxane Laboratories (an extract of Psychotria ipecacuanha used for poison control to induce vomiting).

Thin Layer Chromatography of Evidence Items:

  Evidence Item(s) Analysis Thin Layer Chromatogram
 1 

 A: 001347-06

 B: 001347-07

 C: Emetine (control)

 D: 001347-22

 E: Syrup of Ipecac (control)

Normal Phase thin layer chromatography; mobile phase = 10% Methanol/Chloroform; Anisaldehyde/sulfuric acid developed Anisaldehyde/sulfuric acid developed
 2 

 A: 001347-06

 B: 001347-07

 C: Emetine (control)

 D: 001347-22

 E: Syrup of Ipecac (control)

Normal Phase thin layer chromatography; mobile phase = 10% Methanol/Chloroform; Dragendorff developed Dragendorff developed

Results:

Two methods for developing the chromatograms were used to visualize different chemical compounds. Anisaldehyde is a general reagent used to develop many different types of compounds, while Dragendorff's Reagent is specific for alkaloids.

From the above chromatograms, evidence items 001347-06, 001347-07 and 001347-22 are consistent with the Syrup of Ipecac control.

As well, evidence items 001347-06, 001347-07 and 001347-22 are all positive for an alkaloid consistent with emetine.

Thin layer chromatography was used as a presumptive test, and the presence of emetine in the evidence items has been confirmed using HPLC-MS.

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