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Forensic Evaluation, July 1, 2001
Serology
DNA testing (DQ alpha) was performed on blood samples
submitted to the Crime Lab. Sex determination was accomplished via analysis
at the amelogenin (AMEL) locus. This gene codes for a constituent of human
tooth enamel, the protein amelogenin. The AMEL locus is found on the sex
chromosomes. In females, identical alleles of 106 base pairs (bps) are
found on each X chromosome. In males, slightly different sized alleles
occur: a 106bps allele on the X chromosome and a 112bps allele on the
Y chromosome. This difference in size allows the determination of the
sex of the donor. A sample of DNA obtained from a female will show one
band (representing BOTH X alleles) of 106bps. A sample of DNA from a male
will show two bands: one at 112bps and one at 106bps. Mixtures of samples
will show a variation in band intensity. Theoretically, an equal amount
of male and female DNA will show only two AMEL alleles: one at 106bps
and one at 112bps. However, the band at 106 will be 3X as intense (2 from
female + 1 from male).
DQ Alpha Analysis
Given the nature of the samples, except in the case of known blood samples,
mixed samples are possible. When 3 or more alleles or varying intensities
of AMEL alleles are observed, a mixture is confirmed. However, from this
analysis, it may not be possible to determine the exact number of donors
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DNA
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Evidence Item
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Letter
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Alleles Present
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* = Samples: B, D, I, and L show a definite mixture.
**Presence of allele 1.2 can not be eliminated from this analysis.
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001547-09: One (1) pickaxe, Pick (tapered) end #1
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A
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1.1, 1.3**
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001547-06: One (1) diamond stud earring
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B
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1.1, 1.3, 3**
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001547-10: One (1) Olympus OM-1 35mm camera
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C
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1.1, 1.3**
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001547-09: One (1) pickaxe, Side #1
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D
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1.1, 1.3, 3**
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001547-09: One (1) pickaxe, Axe (flat) end #1
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E
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3, 3
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001547-35: One (1) reddish smear stain, measuring
approximately 1" x 5", Found in bed of University of Mississippi
pickup truck, license plate #UMS 013
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F
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3, 3
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001547-34: One (1) diamond stud earring, Found in
bed of University of Mississippi pickup truck, license plate #UMS
013.
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G
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3, 3
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Blood Sample from Victim Achim Michaelis
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H
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1.1, 1.3
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001547-12-R, right work glove from Tate Moore
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I
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1.2, 3, 4.1
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001547-11: One (1) hedge clipper
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J
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1.2, 4.1
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001547-15-L, left work glove from Cal Swinney
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K
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1.2, 4.1
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001547-15-R, right work glove from Cal Swinney
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L
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2,3
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001547-14-R, right work glove from Lorie Jones
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M
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1.2, 4.1
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