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DNA analysis

NOTE: To assist you in understanding this document, the Crime Lab prepared a tutorial on Multiplex STR DNA analysis.


 

YOKNAPATAWPHA COUNTY FORENSIC LABORATORY

DATE ANALYSIS PERFORMED: 2/15/2012

Madeline Holland, Ph.D.
555 Jackson Avenue
Oxford, MS 38655
662-234-XXXX (FAX 662-234-XXXX)

Analysis: Multiplex STR

Case: 000171-14A-2012

Case Description: Philip Fontaine Homicide

Investigative Agency: Yoknapatawpha County Sheriff's Department

Investigating Officer(s): Det. S. Murphy. Det. T. Armstrong

 

Evidence Item Description

000171-04-02A

Chef's knife ‒ Sample location: 5cm from tip

Electropherogram 000171-04-02A

000171-04-02B

Chef's knife ‒ Sample location: tip of blade

Electropherogram 000171-04-02B

000171-04-02C

Chef's knife ‒ Sample location: handle

Electropherogram 000171-04-02C

000171-04-02D

Chef's knife ‒ Sample location: blade/handle junction

Electropherogram 000171-04-02D

000171-S01

Blood sample from Philip Fontaine

Electropherogram 000171-S01

000171-S02

Presumed blood smear located on Philip Fontaine's left cheek

Electropherogram 000171-S02

000171-S03

Pooled blood near body on kitchen floor

Electropherogram 000171-S03

000171-S04

Presumed bloody fingerprint on kitchen cabinet door

Electropherogram 000171-S04

000171-S05

Shoeprint on kitchen floor

Electropherogram 000171-S05

000171-S06

Red substance on broken glass

Electropherogram 000171-S06

000171-S07

Red droplet on kitchen floor

Electropherogram 000171-S07

000171-S08

Red droplet on kitchen floor

Electropherogram 000171-S08

000171-S09

Red substance on kitchen sink

Electropherogram 000171-S09

Findings:

Items 000171-04-02A and 000171-04-02D are positive mixtures as noted by 3 to 4 peaks at several loci.  By comparing the electropherogram of the victim (000171-S01), items 000171-04-02A and 000171-04-02D contain the DNA of the victim. 

Items 000171-04-02B, 000171-S03, and 000171-S05 can be conclusively matched to the victim (1 in 625,000,000 probability the samples do not belong to the victim). 

Items 000171-04-02C, 000171-S02, 000171-S04, 000171-S06, 000171-S08 and 000171-S09 represent an unknown donor, which has contributed to items 000171-04-02A and 000171-04-02D. (1 in 750,000,000 probability that the unknown donor does not contribute to said items).

Item 000171-S07 represents a third donor inconsistent with the victim or previous unknown donor. 

All unknown donors are male (Amelogenin locus containing 2 peaks).

 

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